1 The choice of solvent to be developed requires not only the development of at least three systems with different polarities, but also the choice of three solvent systems with different intermolecular forces, such as chloroform, methanol, cyclohexane, ethyl acetate, and n-butanol. \Acetic acid\water, respectively, to determine whether the component is a single spot. The advantage of this is that it is clear that by dividing the components by various differences between the components, it is possible that several similar components are a single spot in a solvent system because of the solvent system and the components There is no significant difference in intermolecular force, which is not enough to distinguish TLC. For another solvent system with different intermolecular forces, it is possible to separate. This is impossible to achieve with three different polar unfolding systems.
2 For a solvent system As described by wxw0825, at least three different polarity deployment systems are required. One polar deployment system pushes the Rf of the target component to 0.5, and the other two polar deployment systems target the components. The Rf pushed to 0.8, 0.2. The effect is to check for impurities that are more or less polar than the target component.
3 color development method, light development is not enough, but also use a variety of color methods. Generally, it is necessary to use general-purpose reagents, such as 10% sulfuric acid and iodine, because each reagent (whether it is a general-purpose reagent or a proprietary reagent) has a compound that does not show color in the work. ). Use proprietary color reagents depending on the composition that may contain promiscuous components. Only a single spot under multiple color reagents can only be concluded as a thin layer of pure.